|
KMID : 0545120070170091563
|
|
Journal of Microbiology and Biotechnology
2007 Volume.17 No. 9 p.1563 ~ p.1567
|
|
|
Two Threonine Residues Required for Role of AfsKav in Controlling Morphogenesis and Avermectin Production in Streptomyces avermitilis
|
|
ARISHMA RAJKARNIKAR
Kwon Hyung-Jin
Ryu Yeon-Woo
Suh Joo-Won
|
|
|
Abstract
|
|
|
|
AfsKav is a eukaryotic-type serine/threonine protein kinase, required for sporulation and avermectin production in Streptomyces avermitilis. In terms of their ability complement SJW4001 (afsK-av), afsK-av mutants T165A and T168A were not functional, whereas mutants T165D and T168D retained their ability, indicating that Thr-165 and Thr- 168 are the phosphorylation sites required for the role AfsKav. Expression of the S-adenosylmethione synthetase gene promoted avermectin production in the wild-type S. avermitilis, yet not in the mutant harboring T168D or T165D, demonstrating that tandem phosphorylation on Thr-165 and Thr-168 in AfsKav is the mechanism modulating avermectin production in response to S-adenosylmethione accumulation in S. avermitilis.
|
|
|
KEYWORD
|
|
|
Streptomyces avermitilis, AfsKav, phosphorylation residue, site-directed mutagenesis, avermectin production
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|